1.DirectRNA测序在人类疾病中的研究思路和案例解析随着生命科学领域的技术不断迭代升级,Direct RNA测序(DRS,直接RNA测序)已悄然成为研究者们研究复杂疾病病理机制的强大工具。Direct RNA测序凭借其直接读取全长转录本,无需反转录与PCR扩增的独特优势,可以真实反映细胞内RNA分子的原始状态,精准捕捉RNA单碱基水平上的各类化学修饰,如m6A、m5C和假尿苷等,这些修饰对于转录后https://zhuanlan.zhihu.com/p/694248645

2.CellDiscovery新突破:改良Cas13d实现高效的胞浆RNA靶向治疗研究中开发的核质转运Cas13d能有效将CRISPR RNA(crRNA)从核内转移到细胞质,实现了在细胞质内靶向降解RNA。 在现代生物技术领域,CRISPR/Cas系统已成为基因编辑的重要工具之一。特别是针对RNA的编辑,CRISPR/Cas13系统显示出巨大的潜力。Cas13家族中,Cas13d被认为是哺乳动物细胞中最活跃的亚型。然而,Cas13d在哺乳动物https://news.bioon.com/article/f0ee821e69b9.html

3.科普经典CRISPR/Cas分子诊断系统(一)麻省理工的张锋和加州大学伯克利分校的Jennifer Doudna两位学者不但在CRISPR基因编辑上做出奠基性贡献,也对CRISPR在分子诊断行业的应用做了开拓性工作,开启了CRISPR分子诊断这一全新领域。下面我们就来具体了解下张锋团队的SHERLOCK诊断系统和Jennifer Doudna的DETECTR诊断系统。https://www.kuike-med.com/info-1036-550.html

4.CRISPRdirect:softwarefordesigningCRISPR/CasguideRNACRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNAhttps://xueshu.baidu.com/usercenter/paper/show?paperid=a0058c1af3d6a952798373bd6355cfbd

5.CRISPRdirect:softwarefordesigningCRISPR/CasguideRNACRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNAhttps://www.ncbi.nlm.nih.gov/pubmed/25414360

6.CRISPR验证分析怎么看crisprdirect结果如CRISPR/Cas9 ?通量筛选技术在肿瘤细胞进?阳性或阴性筛选,可以对靶向候选基因向导 RNA 进?富集分析,从中可挖掘肿瘤耐药基因或寻找肿瘤治疗的新靶点。此外sgRNA文库在药物现、基因功能研究、分子诊断、疾病治疗等领域均发挥了重要作用。 应用领域 对于疗效显著但机制不明的药物,可通过药物靶点确定与验证,进行作用https://blog.csdn.net/aganlala/article/details/116200584

7.小麦基因组编辑技术应用中grna设计工具的比较。wheatcrispr(httpsCRISPRdirect网站“Species”的选项多达633个,小麦仅是其中之一的物种,且也只包含“中国春”这一个小麦品种[3](图3)。CRISPRdirect中小麦的参考基因组和识别的PAM序列同E-CRISP,不同之处在于CRISPRdirect设计页面非常简单,用户只需要提交目的序列并选择相应的基因组即可快速获得结果,并没有其他参数可作调整。CRISPRdirehttp://www.360doc.com/content/22/0531/17/68640592_1033995871.shtml

8.文献速递双基因CRISPR敲除策略显著增强实体瘤CAR采用CRISPR-Cas9敲除CAR-T细胞和TCR-T细胞中的Regnase-1 (Reg1-KO) 、Roquin-1 (Roq1-KO) 或两者双敲除 (DKO) 。敲除Regnase-1和/或Roquin-1不影响mesoCAR-T细胞或NYESO TCR-T细胞在生产过程中的扩增速度和转导效率,且操作高效。https://mp.weixin.qq.com/s?__biz=MzA3MzQyNjY1MQ==&mid=2652780318&idx=2&sn=90f822179fddae9a4f12256ab3e7901b&chksm=85e80bb6ffb5a8974d2172bbb36836a7da8f4e20a4c77f2461d56097a0541ec69bf9f2785d32&scene=27

9.CRISPRThe elegance of CRISPR lies in its simplicity, needing just two components: the sgRNA and a Cas protein [39]. This streamlined design makes it straightforward to program and direct towards genomic areas of interest [39]. Its broad range of applicability, spanning from human cells to bacterial https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/crispr

10.MetaCRAST:referenceMetaCRAST(Metagenomic CRISPR Reference-Aided Search Tool) is a tool to detect CRISPR arrays in raw, unassembled metagenomes. Unlike other tools, it uses expected CRISPR direct repeat (DR) sequences from assembled contigs or bacterial genomes to guide metagenomic CRISPR detection. It uses a fast https://github.com/molleraj/MetaCRAST

11.ofBaredSpCas9,ScaffoldgRNA,andTypeIIAnticerevisiae genome was verified with CRISPRdirect. (34) Therefore, under the guidance of sgRNA (in this study, if not otherwise specified, sgRNA is supposed to bind the antisense strand of the target DNA), dSpCas9-AD:sgRNA bound the synthetic promoter and then activated transcription by https://pubs.acs.org/doi/full/10.1021/acssynbio.1c00352

12.CRISPRThe CRISPRCasFinder program enables the easy detection of CRISPRs and cas genes in user-submitted sequence data.https://crisprcas.i2bc.paris-saclay.fr/

13.CRISPRPAM 一起长度为 23nt ,其中种子序列含9 个碱基, 脱靶位点的PAM 类型为“NGG”或“NAG” ;(3)sgRNA 2.1.10 CRISPRdirect 和PAM 一起长度为23nt ,其中种子序列含 10 个碱 [35] 该软件 可设计特异性的sgRNA ,其提供有人、 基,脱靶位点的 PAM 类型为“NGG”或“NAG”(默认 小鼠、大鼠和猪等 18 个https://max.book118.com/html/2017/0920/134497738.shtm

14.EvolutionandClassificationofCRISPRBiol Direct 4:29PubMedCrossRefGoogle Scholar Marraffini LA, Sontheimer EJ (2009) Invasive DNA, chopped and in the CRISPR. Structure 17:786–788PubMedCrossRefGoogle Scholar Mulepati S, Bailey S (2011) Structural and biochemical analysis of the nuclease domain of the clustered regularly http://doi.org/10.1007/978-3-642-34657-6_3

15.采用CRISPRi原位探索大肠杆菌Nissle1917毒素为进一步探索TA系统对于EcN 1917益生特性的调控机制,在此我们采用CRISPRi技术进行了原位敲低试验。依据mazEF,hipA基因序列,采用CRISPRdirect设计并合成gRNA片段,与载体连接后构建了转化质粒;再分别引入mazEF-gsRNA/dCas9和hipA-gsRNA/dCas9质粒。EcN 1917转化子EcN-AmazE与EcN-△hipA经诱导后,RT-PCR检测及PAGE试验https://wap.cnki.net/lunwen-1019091463.html

16.FrontiersCRISPR/CasgenomeeditingintomatoimprovementCRISPRdirect (https://crispr.dbcls.jp), CRISPR-P (http://cbi.hzau.edu.cn/cgi-bin/CRISPR), CRISPR-PLANT (http://omap.org/crispr/index.html), CRISPR-GE (http://skl.scau.edu.cn/), Breaking-Cas (http://bioinfogp.cnb.csic.es/tools/breakingcas/), CRISPOR.org (http://crispor.org)https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1121209/full

17.NanomaterialsThe most effective strategy for utilizing CRISPR/Cas9 system is the direct delivery of Cas9 protein and sgRNA. This direct approach bypasses transcription and translation process, resulting in rapid gene editing and enhanced application potential [168]. Incubation of Cas9 protein and sgRNA in vitro prhttps://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02627-w

18.哈佛专家论述CRISPR检测技术的开发与应用以及发展方向该篇概述了如何将不同的CRISPR Cas酶的特性用于诊断检测,探讨了不同的预扩增策略(目前构成大多数基于CRISPR的诊断检测的一部分),研究定量和多路复用的方法,重点介绍结合 CRISPR 的技术-基于测序的目标富集,并审查针对 POC 应用的基于 CRISPR 的诊断的优化,重点是检测读数和样品制备。文章还概述了该技术的新兴生物医学https://m.magigen.com/cn/CRISPR-detection-technology-review-and-outlook.html

19.CRISPRCas9基因敲除大肠杆菌BL21菌种中的lacZ基因技术中心通过CRISPR/Cas9编辑β--半乳糖苷酶基因,造成β-半乳糖苷酶基因功能缺失,编辑后的BL21菌种将不能代谢X-gal产生蓝斑。 实验材料 大肠杆菌Cas9基因编辑试剂盒(英茂盛业,CR3011) Clone Direct PCR Mix(英茂盛业,P3001) 靶点和实验方案设计 根据BL21中β-半乳糖苷酶基因序列,设计基因敲除靶点和重组修复模板。 设计https://www.inovogen.com/news/2016/1230/442.html

20.手把手教你学会CRISPRCas9基因敲除技术使用在线设计网站 CRISPR direct,如需直接复制网址,可在生物学霸后台对话框回复 direct即可。 靶点挑选要点: 基因敲除靶点应设计在起始密码子附近(包括起始密码子)或者起始密码子下游的外显子范围内。 不同Cas9/gRNA 靶点在基因敲除效率上有较大差异,因此同时设计构建 2～3 个靶点的基因敲除载体再从中选出敲减效果https://www.biomart.cn/experiment/793/2713886.htm

21.Addgene:CRISPRReferencesandInformationOriginally developed to identify zinc finger nuclease sites, this tool from theJoung Labhas been expanded to identify potential DNA target sites for TALEs and CRISPR/Cas. CRISPRdirect From the Database Center for Life Science (DBCLS) in Japan; Identify candidate gRNA target sequences in an inputhttp://www.addgene.org/crispr/reference/

22.MolecularCellTypeI1. Klompe, S.E., Vo, P.L.H., Halpin-Healy, T.S., and Sternberg, S.H. (2019). Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integra- tion.Nature571, 219–225. 2. Petassi, M.T., Hsieh, S.C., and Peters, J.E. (2020). Guide RNA categorization enables targethttps://weibo.com/ttarticle/p/show?id=2309404734539380687403

23.CRISPR2018–June20Please note this is a preliminary programme, which could undergo minor changes. June 20June 21June 22June 23 June 20, Wednesday The conference fee includes access to the conference, its materials, lunches and the conference dinner on Friday (but NOT accommodations or the Saturday trip to Trakaihttp://www.crispr2018.gmc.vu.lt/

24.CRISPRCas13aAntibodyMAB10462All CRISPR-Cas13a Antibodies Product Details FAQs Supplemental Products Reviews Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas CRISPR-Cas13a Antibody Summary Specificity Detects CRISPR-Cas13a in direct ELISAs. Source Monoclonal Mouse IgG1 https://www.rndsystems.com/cn/products/crispr-cas13a-antibody-1020221_mab10462

25.CRISPRCas9GeneEditingCRISPRCas9NewsArticlesWe are your one-stop resource for all your CRISPR needs.CAS9 April 30, 2022 What is CRISPR Being Used For? CRISPR is a revolutionizing technology that can be used for gene editing, and as a direct result, has the potential to change the world as we know it. The essence of CRISPR-https://cas9.com/

26.CRISPR【1】 Song G, Jia M, Chen K, et al. ScienceDirect CRISPR / Cas 9: A powerful tool for crop genome editing. The Crop Journal. 2016. 【2】Jinek, M.; East,A.; Cheng, A.; Lin, S.; Ma, E.; Doudna, J. RNA-programmed genomeediting in human cells. eLife. 2013. 2,e00471. dohttps://www.aptbiotech.com/market/6157